北京义翘神州 多次跨膜蛋白开发 三大技术平台助力研发 蛋白药物研发

VLP技术平台

去垢剂技术平台

Nanodisc技术平台

高活性

Immobilized Human GPRC5D-VLP (Full Length) Protein at 5 μg/mL (100 μL/well) binds anti-GPRC5D antibody.

高批间一致性

Immobilized Human GPRC5D-VLP (Full Length) Protein at 5 μg/mL (100 μL/well) binds anti-GPRC5D antibody.

Immobilized Human GPRC5D-Detergent Protein (His & FLAG Tag) at 5 μg/mL (100 μL/well) on Nickel coated plate binds anti-human GPRC5D antibody.

Loaded anti-GPRC5D antibody on Protein A Biosensor binds Human GPRC5D-Detergent Protein (His & FLAG Tag) with an affinity constant of 58.1 nM as determined in BLI assay (ForteBio Octet Red384).

Immobilized Human GPRC5D-Nanodisc Protein (His & FLAG Tag) at 5 μg/mL (100 μL/well) can bind anti-Human GPRC5D antibody.

Loaded anti-GPRC5D antibody on Protein A Biosensor binds Human GPRC5D-Nanodisc Protein (His & FLAG Tag) with an affinity constant of 0.782 nM as determined in BLI assay (ForteBio Octet Red384).

Captured anti-GPRC5D antibody on anti-Human IgG Fc via CM5 Chip binds Human GPRC5D-Nanodisc Protein (His & FLAG Tag) with an affinity constant of 9.609 nM as determined in a SPR assay (Biacore T200).

Human Claudin 6-VLP Protein  |  Cat#: 16658-HNAH  

Immobilized human claudin 6 protein can bind anti-claudin6 antibody

Freeze-thaw stability

Batch-to-batch Consistency

 

Immobilized human GPRC5D protein can bind anti-GPRC5D antibody  

Immobilized human SSTR2 / SSTR2A protein can bind anti-SSTR2 antibody  

Immobilized human SSTR2 / SSTR2B protein can bind anti-SSTR2 antibody

Immobilized human CXCR4/CD184 protein can bind anti-CXR4/CD184 antibody

VLP平台

Detergent平台

SMA-Nanodisc平台

我想购买膜蛋白产品做免疫和抗体筛选，应该怎么挑选？

目前，我们的3个平台的产品各有特点，客户可以按照实际情况进行选择。VLP平台产品是一个混合物，与细胞免疫相比靶标蛋白的丰度有所提高，在没有其他平台的产品时可以用于免疫和抗体筛选。由于Detergent平台的产品存在去垢剂，因此，需要考虑去垢剂对于免疫动物的毒害和免疫过程中蛋白变性的风险。比较而言，SMA-Nanodisc平台的产品既拥有较高的靶蛋白丰度，又能维持好的天然构象，特别推荐用于动物免疫和抗体筛选实验。