绿色3CLglow 3CLpro测定-Green 3CLgl

绿色3CLglow 3CLpro测定，Green 3CLglow 3CLpro assay，绿色3CLglow 3CLpro测定,Green 3CLglow 3CLpro assay

产品名称：未标记神经纤毛蛋白-1 BacMam-Green 3CLglow 3CLpro assay

产品货号：MTA-C1160G-005

产品规格：5mL

测量3CL蛋白酶（3CLpro）抑制或用活细胞荧光3CL glow测定筛选抑制剂。3CLglow传感器在存在3CLpro酶的情况下表达绿色荧光蛋白以及组成型表达的红色荧光蛋白，以监测细胞健康和化合物毒性。该检测试剂盒包装在BacMam中，用于在哺乳动物细胞中表达。BacMam在HEK293细胞中提供甚至接近100%的转导效率，以及在许多其他细胞系、iPSC和原代培养物（包括A549和HUVEC）中的表达。表达可根据您的实验需要轻松滴定或调整检测灵敏度。 该试剂盒包括3CLglow传感器BacMam、3CLpro酶BacMam和500mM丁酸钠水溶液。

Measure 3CL protease (3CLpro) inhibition or screen for inhibitors with the live cell, fluorescent 3CLglow assay. The 3CLglow sensor expresses a green fluorescent protein in the presence of the 3CLpro enzyme as well as a constitutively expressed red fluorescent protein to monitor cell health and compound toxicity. The assay is packaged in BacMam for expression in mammalian cells. BacMam provides even, near 100% transduction efficiency in HEK293 cells as well as expression in many other cell lines, iPSCs, and primary cultures including A549s and HUVECs. Expression is easily titrateable to your experimental needs or to adjust assay sensitivity. This kit includes the 3CLglow sensor BacMam, 3CLpro enyme BacMam, and 500mM sodium butyrate in H2O.

4°C.

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