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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
α-Aminoisobutyric acid
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
A425173-1ml
英文名:α-Aminoisobutyric acid
纯度或规格:10mM in Water
SPU:A425173
CAS:62-57-7
存储温度:-80℃储存
运输条件:特低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
METHOD Protocol 1: Preparation of OP9 Cells for Co-culture Thawing OP9 or OP9-DL1 Cells 1. Add 12 mL of OP9 medium to a 15-mL centrifuge tube. 2. Thaw OP9 or OP9-DL1 cells quickly in a 37°C water bath.
, 2.2M dNTP’s 100mM EDTA 0.5M EtBr 10mg/ml Gelatin 2% Glucose 1; 1.5; 2M Guanidine HCl 1-8M HEPES 1M Imidazol 2M Paraformaldehyde 37% PEG 40% PMSF 100mM Retinoic acid 10mM Sucrose 1; 2; 2.5M Tris Cl 1M
Construction of Gene-Targeting Vectors by Recombineering
: Multimers of plasmids are obtained after recombineering. [Steps 21, 32, and 57] Solution: Follow Steps 57-62 and perform selection using appropriate antibiotics after retransformation. Alternatively, choose a suitable restriction enzyme
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