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- 文献和实验
- 技术资料
- 库存:
期货
- 规格:
F477501-100mg
中文名:F.A.M.E. Mix RM-5
英文名:F.A.M.E. Mix RM-5
纯度:分析标准品,ampule of 100mg (Neat)
货号:F477501
存储温度:-20°C储存
运输条件:超低温运输
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文献和实验to laboratory in a sealed container. Still wearing protective clothing, remove ampule from container and place in a waterbath at an appropriate temperature for your cell line e.g. 37oC for mammalian cells. Submerge only the lower half of the ampule. Allow
CTAB TECHNIQUE / Method / Sched
8) + 2.5 vol cold 100% ethanol. 12. Spool DNA out. Air dry and resuspend in in 0.5 to 1ml T.E. (takes time) and freeze until required. DNA Quantification Make a 0.8% agarose gel with 1x TAE and 0.1µl of Ethidium bromide per 10ml solution). Load samples neat
isopropanol - can leave overnight. Spool out or spin down DNA, 2min at 2,000rpm. 8. Transfer to 5ml wash buffer for 20min. 9. Dry briefly and resuspend in 1ml T.E. (can be left overnight) 10. Add 1µl 10mg/ml RNAse to each 1ml T.E./DNA
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