- ￥166.90
- 阿拉丁
- 上海
- P114538
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
避光
- 英文名：
Phenthoate solution
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- CAS号：
13376-78-8
- 规格：
P114538-1ml
溶于正乙烷、乙二醇二乙酯。在酸性、中性介质中稳定。
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文献和实验Yeast IF with Methanol/Acetone Dehydration
containing 1.2M sorbitol. Be very gentle with the cells! Resuspend in 0.5 mls sorbitol buffer. The cells can be stored at this point overnight at 4¡C. Zymolyase treat the cells with 30 ul 10 mg/ml Zymolyase (100T) at 30¡C for anywhere between 10-30 min
FIXATION WITH FORMALDEHYDE-ACETONE
out. 8. Proceed with staining. 9. Used acetone should be emptied into a designated container. 10. Note: The acetone step may be replaced by adding 0.1% Triton X-100 to solution 4 and do fixation and extraction in one step.
Yeast IF with Methanol/Acetone Dehydration
in 0.5 mls sorbitol buffer. The cells can be stored at this point overnight at 4¡C. Zymolyase treat the cells with 30 ul 10 mg/ml Zymolyase (100T) at 30¡C for anywhere between 10-30 min or more. Examine cells on a phase microscope. When cells are dark
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