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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
室温
- 英文名:
Poly(dimethylsiloxane)
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
9016-00-6
- 规格:
P195721-5kg/P195721-50g/P195721-250g/P195721-1kg
| 规格: | P195721-5kg | 产品价格: | ¥4087.9 |
|---|---|---|---|
| 规格: | P195721-50g | 产品价格: | ¥95.9 |
| 规格: | P195721-250g | 产品价格: | ¥367.9 |
| 规格: | P195721-1kg | 产品价格: | ¥958.9 |
英文名:Poly(dimethylsiloxane)
纯度或规格:average M.W. 115,000
SPU:P195721
CAS:9016-00-6
存储温度:室温
运输条件:常规运输
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文献和实验(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl
Preparation of End-Labeled DNA Probes by Conventional Kinase for DNA Footprinting Analysis
(see Hint #11). 8. Incubate at -80°C for between 30 min to overnight. 9. Centrifuge at 115,000 X g for 20 min at 4°C (35,000 rpm using an SW 50.1 rotor). 10. After centrifugation, remove the supernatant and vacuum dry
Practical Aspects of Radioligand Binding
with intact N1E‐115 cells (300,000 cells/tube) for 45 min at 37°C, at which time equilibrium is reached. The dissociation of radioligand from the receptors was followed for various periods of time after the addition of 10 µM NMS, and the reactions terminated
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