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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein of human RRM2 (AA 5-130)
- 亚型:
Rabbit IgG
- 保存条件:
-20°C储存,避免反复冻融;超低温运输
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 浓度:
0.2 mg/mL
- 规格:
Ab126016-100μl/Ab126016-1ml/Ab126016-50μl/Ab126016-10μl
| 规格: | Ab126016-100μl | 产品价格: | ¥2099.90 |
|---|---|---|---|
| 规格: | Ab126016-1ml | 产品价格: | ¥16999.90 |
| 规格: | Ab126016-50μl | 产品价格: | ¥1299.90 |
| 规格: | Ab126016-10μl | 产品价格: | ¥499.90 |
基本信息
产品名称:RRM2 Antibody
规格或纯度:ExactAb™, Validated, 0.2 mg/mL
应用:WB
特异性:RRM2
种属反应性:人(Human)
免疫原:Recombinant protein of human RRM2 (AA 5-130)
阳性对照:WB: HeLa and A375 cell lysates.
偶联:Unconjugated
产品属性
克隆类型:多克隆抗体
Format:Whole IgG
亚型:Rabbit IgG
SDS-PAGE:150 kDa
纯化方法:Protein A purified
物理外观:Liquid
储存缓冲液:10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂:0.02% Sodium azide
浓度:0.2 mg/mL
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4°C 短期储存(1-2 周)。-20°C下长期保存(24个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human)
偶联:Unconjugated
图片
RRM2 Antibody (Ab126016) - Western Blot
All lanes: RRM2 Antibody (Ab126016) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 43 kDa
Exposure time: 104.9 s
RRM2 Antibody (Ab126016) - Western Blot
All lanes: RRM2 Antibody (Ab126016) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 45 kDa
Observed band size: 43 kDa
Exposure time: 10.0 s
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文献和实验chamber (see note 3); Wash at least 3 times in 1X PBS for 5 minutes each; DNA counterstaining (2 minutes in 1X PBS containing 0.2mg/ml DAPI); Wash twice in 1X PBS; Mount coverslip on a slide in glycerol based mounting medium
Fluorescent in situ Hybridization Combined with Immunostaining on Polytene Chromosomes
solution 2 3.7% paraformaldehyde, 1% Triton X-100, in PBS (37% p-formaldehyde stock solution: weigh 1.85g of p-formaldehyde powder. Add in H2 O (final volume of 5ml), add 70µl 1 N KOH, dissolve by boiling). solution
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
analysis. Restriction enzyme buffer (10X) Dilute the 10X stock to 1.14X for Steps 12 and 13. RNase A, DNase-free (10 mg/mL; Sigma R6513) SDS (Sodium dodecyl sulfate; 20% w/v; Fisher BP166) Sodium acetate (3 M, pH 5.2
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