- ¥374.90 - 16999.90
- 阿拉丁
- 上海
- Ab115170
- 2026年09月16日
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- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide derived from human METTL3 (AA 1-285)
- 亚型：
Rabbit IgG
- 保存条件：
-20°C储存,避免反复冻融；超低温运输
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- 浓度：
见COA
- 规格：
Ab115170-100μl/Ab115170-1ml/Ab115170-50μl/Ab115170-10μl
| 规格： | Ab115170-100μl | 产品价格： | ¥1574.90 |
|---|---|---|---|
| 规格： | Ab115170-1ml | 产品价格： | ¥16999.90 |
| 规格： | Ab115170-50μl | 产品价格： | ¥974.90 |
| 规格： | Ab115170-10μl | 产品价格： | ¥374.90 |
基本信息
产品名称：Recombinant METTL3 Antibody
规格或纯度：重组, ExactAb™, 已验证, 见COA
应用：WB, IP
特异性：METTL3
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
免疫原：A synthetic peptide derived from human METTL3 (AA 1-285)
阳性对照：WB: HEK293, Raji, HeLa, Hep G2, RAW 264.7 and C6 cell lysates.
偶联：Unconjugated
产品属性
克隆类型：重组抗体
Format：Whole IgG
亚型：Rabbit IgG
轻链亚型：kappa
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：10mM PBS, 150mM NaCl, 50% Glycerol, 0.05% BSA, 0.02% Sodium azide, pH 7.4
防腐剂：0.02% Sodium azide
浓度：见COA
储存条件：-20°C储存,避免反复冻融
运输条件：超低温运输
稳定性与储存：短期储存2-8℃（1-2周）；长期储存-20℃（24个月）；收货后建议分装，避免反复冻融。
功能和特点
宿主种属：兔(Rabbit)
种属反应性：人(Human), 小鼠(Mouse), 大鼠(Rat)
偶联：Unconjugated
图片
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Recombinant METTL3 Antibody (Ab115170) - Western Blot
All lanes: Recombinant METTL3 Antibody (Ab115170) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/40000 dilution
Predicted band size: 64 kDa
Observed band size: 71 kDa
Exposure time: 12.1 s
>
Recombinant METTL3 Antibody (Ab115170) - Western Blot
All lanes: Recombinant METTL3 Antibody (Ab115170) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 64 kDa
Observed band size: 71 kDa
Exposure time: 0.3 s
>
Recombinant METTL3 Antibody (Ab115170) - IP
Immunoprecipitate (IP) analysis using the Recombinant METTL3 Antibody (Ab115170) at 1/50 dilution. (WB with Recombinant METTL3 Antibody (Ab115170) at 1/3000 dilution)
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文献和实验Antibody Validation by Western Blotting
must be thoroughly demonstrated using a complex biological sample, rather than a recombinant protein, prior to use in clinical translational research. In the simplest iteration, antibody specificity is determined by the presence of a single band in a complex
Production of Monoclonal Antibody by DNA Immunization with Electroporation
associated with problematic antigens: low abundance and protein instability and use of recombinant proteins that lack posttranslational modifications. This chapter describes the use of a DNA-based immunization protocol for the production of mAb
VERIFY overexpression lysate antigen standards （Lane 1 to 7） were fractionated on SDS-PAGE, and then immunoblotted with 8F1 （Upper panel）。 The recombinant protein expression levels within the lysates were analyzed with anti-DDK antibody （Lower panel
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