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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein of human CTGF (AA 27-349)
- 亚型:
Rabbit IgG
- 保存条件:
-20°C储存,避免反复冻融;超低温运输
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 浓度:
见COA
- 规格:
Ab098051-100μl/Ab098051-1ml/Ab098051-50μl/Ab098051-10μl
| 规格: | Ab098051-100μl | 产品价格: | ¥2099.90 |
|---|---|---|---|
| 规格: | Ab098051-1ml | 产品价格: | ¥16999.90 |
| 规格: | Ab098051-50μl | 产品价格: | ¥1299.90 |
| 规格: | Ab098051-10μl | 产品价格: | ¥499.90 |
基本信息
产品名称:Recombinant CTGF Antibody
规格或纯度:重组, ExactAb™, 已验证, 见COA
应用:WB
特异性:CTGF
种属反应性:人(Human), 小鼠(Mouse)
免疫原:Recombinant protein of human CTGF (AA 27-349)
阳性对照:WB: C6, HUVEC and Hep G2 cell lysates.
偶联:Unconjugated
产品属性
克隆类型:重组抗体
Format:Whole IgG
亚型:Rabbit IgG
轻链亚型:kappa
SDS-PAGE:150 kDa
纯化方法:Protein A purified
物理外观:Liquid
储存缓冲液:50mM Tris-Glycine, 150mM NaCl, 40% Glycerol, 0.05% BSA, 0.01% Sodium azide, pH 7.4
防腐剂:0.01% Sodium azide
浓度:见COA
储存条件:-20°C储存,避免反复冻融
运输条件:超低温运输
稳定性与储存:4℃ 短期储存(1-2 周)。-20℃长期保存(24 个月)。收货后建议分装。避免冷冻/解冻循环。
功能和特点
宿主种属:兔(Rabbit)
种属反应性:人(Human), 小鼠(Mouse)
偶联:Unconjugated
图片
Recombinant CTGF Antibody (Ab098051) - Western Blot
All lanes: Recombinant CTGF Antibody (Ab098051) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution
Predicted band size: 38 kDa
Observed band size: 18, 33 kDa
Exposure time: 40.5 s
Recombinant CTGF Antibody (Ab098051) - Western Blot
All lanes: Recombinant CTGF Antibody (Ab098051) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution
Predicted band size: 38 kDa
Observed band size: 18, 33 kDa
Exposure time: 150.0 s
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文献和实验Antibody Validation by Western Blotting
must be thoroughly demonstrated using a complex biological sample, rather than a recombinant protein, prior to use in clinical translational research. In the simplest iteration, antibody specificity is determined by the presence of a single band in a complex
Production of Monoclonal Antibody by DNA Immunization with Electroporation
associated with problematic antigens: low abundance and protein instability and use of recombinant proteins that lack posttranslational modifications. This chapter describes the use of a DNA-based immunization protocol for the production of mAb
VERIFY overexpression lysate antigen standards (Lane 1 to 7) were fractionated on SDS-PAGE, and then immunoblotted with 8F1 (Upper panel)。 The recombinant protein expression levels within the lysates were analyzed with anti-DDK antibody (Lower panel
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