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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
避光;-20°C储存
- 英文名:
Vitamin D₃ solution
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
67-97-0
- 规格:
V433297-1ml
英文名:Vitamin D₃ solution
纯度或规格:Moligand™, ≥97%, 100 μg/mL in ethanol
SPU:V433297
CAS:67-97-0
存储温度:避光;-20°C储存
运输条件:超低温运输
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文献和实验containing chloramphenicol (Cm, 20 μg/ml)/streptomycin (Stp, 75 μg/ml) and purified using the Large construct kit (Qiagen, Valencia, CA, USA). PCR PCR reactions using oligonucleotides carrying HAs were performed using
Procedure for GenEluteTM Plasmid Miniprep Kit(质粒小量
for 1 to 2 minutes without any additional Wash Solution to remove excess ethanol. 8. Elute DNA Transfer the column to a fresh collection tube. Add 100 %26micro;l of Elution Solution or molecular biology reagent water to the column. For DNA sequencing
(5 µL DNA, + 1 µL enzyme, + 1.5 µL 10x buffer, + 7.5 µL H2 O) 37ºC x 1-2 hrs. Run on 0.8% agarose gel (in 0.5x TBE gel + 0.5 µg/mL ethidium Br) x 2 hrs, to check DNA quality. B. Cells: Pellet cells and wash 1x in PBS. Optional:
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