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上海阿拉丁生化科技股份有限公司
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文献和实验13 Synthesis of Peptide Bioconjugates
and oligopeptides as epitopes with small molecular mass. The synthetic procedures outline three major strategies for the incorporation of multiple copies of uniformly oriented peptide epitopes. In the first example, chloroacetylated polypeptide is conjugated with SH
Using Multiple Tracers for 13C Metabolic Flux Analysis
for performing 13 C-MFA studies using multiple isotopic tracers, a technique that is especially useful for elucidating fluxes in complex biological systems where multiple carbon sources are present. Here, as an example, we describe key steps and decision points
This chapter introduces 13 C tracer techniques combined with labeling measurement by mass spectrometry (MS) for the quantification of metabolic pathway fluxes. As an example, the relative contribution of (1) de novo synthesis from glucose
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