Stearic acid PEG Folate，MW 340

(转贴)核酸、核苷酸的基本换算

,502 bp) = 0.06 pmol ends 2．核苷酸的换算： (1)分子量： MW (Da) = 333 × N (number of bases) (2)浓度： C (μmol/L or pmol/ml) = OD260 / (0.01 × N) C (ng/ml) = OD260´ MW / (0.01 × N) MW -- molecular weight N -- number of bases OD260 -- absorbance at 260 nm

The TRAFO Solutions

should be between 8.4 - 8.9. Note : Lithium Acetate can be sterilized in the autoclave for 20 mins liquid cycle.     Polyethylene glycol (PEG 50% w/v) The polyethylene glycol (PEG), MW 3350 (Sigma P3640) is made up to 50% (w/v) with dd water and filter

单组分溶液配制

, 2.2M dNTP’s 100mM EDTA 0.5M EtBr 10mg/ml Gelatin 2% Glucose 1; 1.5; 2M Guanidine HCl 1-8M HEPES 1M Imidazol 2M Paraformaldehyde 37% PEG 40% PMSF 100mM Retinoic acid 10mM Sucrose 1; 2; 2.5M Tris Cl 1M