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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
避光;-20°C储存;充氩
- 英文名:
NHS-PEG-NHS
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
N164055-50mg/N164055-250mg/N164055-1g
| 规格: | N164055-50mg | 产品价格: | ¥109.9 |
|---|---|---|---|
| 规格: | N164055-250mg | 产品价格: | ¥385.9 |
| 规格: | N164055-1g | 产品价格: | ¥1109.9 |
N-hydroxylsuccinimide (NHS) functionalized polyethylene glycol (PEG-NHS) is an amino (-NH2) reactive PEG derivative that can be used to modify protein, peptide or any other surfaces with their available amino groups. NHS esters react with primary amine groups at pH 7~8.5 to form stable amide bonds.Compared to other PEG NHS ester derivatives, our succinimidyl carbonate (SC) functionalized mPEG-NHS offers superior reactivity and higher stability in aqueous solution. NHS esters react with deprotonated primary amines, therefore, the reaction requires neutral to basic pH values to proceed. Primary amines react with NHS esters by nucleophilic attack and NHS is released as a byproduct. Hydrolysis of the NHS-ester competes with the reaction in aqueous solution and increases with increasing pH.
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文献和实验by microcentrifugation at ≥ 8000 x g for 1-2 minutes.25.Repeat steps 23. and 24. This is a total of two washes with 1 mL PBS-TBN.26.Remove the supernatant and resuspend the coupled and washed microspheres in 250-1000 μL of PBS-TBN. 27.Count the microsphere
International (Cupertino, CA). BSA-NHS slides, displaying activated amino and carboxyl groups on the surface of an immobilized layer of bovine serum albumin (BSA), were fabricated as follows. 10.24 g N,N'-disuccinimidyl carbonate (100 mM) and 6.96 ml N,N
,502 bp) = 0.06 pmol ends 2.核苷酸的换算: (1)分子量: MW (Da) = 333 × N (number of bases) (2)浓度: C (μmol/L or pmol/ml) = OD260 / (0.01 × N) C (ng/ml) = OD260´ MW / (0.01 × N) MW -- molecular weight N -- number of bases OD260 -- absorbance at 260 nm
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