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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存
- 英文名:
Lipopolysaccharides from Pseudomonas aeruginosa 10
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
L611586-5mg/L611586-1mg/L611586-10mg
| 规格: | L611586-5mg | 产品价格: | ¥999.9 |
|---|---|---|---|
| 规格: | L611586-1mg | 产品价格: | ¥599.9 |
| 规格: | L611586-10mg | 产品价格: | ¥1599.9 |
本品为绿脓杆菌血清型10提取的 TCA。来源菌株为 ATCC 27316。纯化方式;trichloroacetic acid extraction
应用:
脂多糖 (LPS) 是革兰氏阴性菌细胞壁的特征性成分。LPS 及其脂质 A 部分通过 Toll 样受体 4 (TLR4) 刺激先天免疫系统的细胞,TLR4 是 Toll 样受体蛋白家族的成员之一,可识别常见的病原体相关分子模式 (PAMP)。
制备说明:
本品可溶于水(5 mg/mL)或细胞培养基(1 mg/mL),产生淡黄色浑浊溶液。涡旋并加热至 70-80 o C 后,在盐水中获得了更浓缩但仍浑浊的溶液(20 mg/mL)。脂多糖是在每种溶剂中形成胶束的分子。在水和磷酸盐缓冲盐水中观察到浑浊溶液。有机溶剂不能提供更清晰的溶液。甲醇产生浑浊、带漂浮物的混悬液,而水产生均匀的浑浊溶液。
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文献和实验Genomic DNA Extraction - PureLink
without ethanol precipitation or any additional steps. General guidelines for PFGE are described below. For details, refer to the manufacturer’s recommendations. For PFGE, load 20 µl (0.5-1 µg) purified DNA/lane in 10X BlueJuice™ Gel loading Buffer on a 1
Methods for the Detection of D-Amino-Acid Oxidase
on the activity of the samples. It was terminated by the addition of 1 ml of 10% trichloroacetic acid. In a blank, trichloroacetic acid was added to the reaction mixture before the enzyme reaction was started. The precipitate was removed by centrifugation (700 x g
Methods for the Detection of D-Amino-Acid Oxidase
on the activity of the samples. It was terminated by the addition of 1 ml of 10% trichloroacetic acid. In a blank, trichloroacetic acid was added to the reaction mixture before the enzyme reaction was started. The precipitate was removed by centrifugation (700 x g
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