WAY-606724，519049-15-1，10mM in

Competent Cell Preparation

and autoclave to sterilize.  2. TB solution:  *10mM PIPES  *15mM CaCl 2 (1.66g/L) *250mM KCl(18.6g/L) *Dissolve in nanopure water and adjust pH to 6.7 with KOH or HCl and then add MnCl 2  to 55mM

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

gr/l Difco bacto agar. Hypoclorite solution: for 50 ml: 4ml Na Hypoclorite 15% 255l Tween-20 water to 50ml   cDNA PROBES mRNA 1-5ug H2O 50ul oligoDt (10mg/ml) 2ul heat 90C 2'', ice 5xRT buffer 20 10mM

In Situ HYBRIDIZATION TO TISSUE SECTIONS

DNase (1U/ml) to the 24ml reaction mixture and incubate 30' @ 37 degrees C. Stop the reaction: +60ml 10mM EDTA, 10mM Tris-HCl (pH 7.4), 0.2% SDS +1.67ml 5M NaCl +1.0ml 1M DTT Incubate 10-15' on ice Hydrolyze the probe (ideal