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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
避光;-20°C储存;干燥
- 英文名:
MeCY5-NHS ester triethylamine
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
M658686-5mg/M658686-50mg/M658686-25mg/M658686-10mg
| 规格: | M658686-5mg | 产品价格: | ¥1200.9 |
|---|---|---|---|
| 规格: | M658686-50mg | 产品价格: | ¥6200.9 |
| 规格: | M658686-25mg | 产品价格: | ¥3850.9 |
| 规格: | M658686-10mg | 产品价格: | ¥1920.9 |
英文名:MeCY5-NHS ester triethylamine
纯度或规格:≥96%
SPU:M658686
CAS:-
存储温度:避光;-20°C储存;干燥
运输条件:超低温运输
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文献和实验Coupling Peptides To Resin For Affinity Purification
5% ethylene diamine in water. Incubate 15' at RT. Wash with 10 volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate
Coupling Peptides To Resin For Affinity Purification
volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add
Revised amino-allyl labeling protocol
, all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cy-dyes from coupling to free amine groups in solution. 3. Add 450 m L water to each reaction. C. Cleanup Add 500 m L neutralized, diluted reaction
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