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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-80℃储存
- 英文名:
Dydrogesterone
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
152-62-5
- 规格:
D580377-1ml
英文名:Dydrogesterone
纯度或规格:Moligand™, 10mM in DMSO
SPU:D580377
CAS:152-62-5
存储温度:-80℃储存
运输条件:特低温运输
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文献和实验Northern Blot: Glyoxal/DMSO Adapted from Molecular Cloning (Maniatis), Current Protocols in Molecular Biology, and J. Yost Lab (UMN) Objective: Glyoxal/DMSO gels are great for determining sizes of RNA especially in conjunction
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
In Situ HYBRIDIZATION TO TISSUE SECTIONS
dechorionated embryos to either buffer A or B (depending on their age): Buffer A (0-2, 1-3 hr embryos) 2.5 ml 8% paraformaldehyde (fresh) 2.0 ml 1X PBS 0.5 ml DMSO - or - Buffer B (2-4, 3-5
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