- ￥943.90
- 阿拉丁
- 上海
- T408410
- 2025年12月31日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-80℃储存
- 英文名：
Trimethoprim
- 库存：
现货
- 供应商：
上海阿拉丁生化科技股份有限公司
- CAS号：
738-70-5
- 规格：
T408410-1ml
英文名：Trimethoprim
纯度或规格：Moligand™, 10mM in DMSO
SPU：T408410
CAS：738-70-5
存储温度：-80℃储存
运输条件：特低温运输
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文献和实验Northern Blot: Glyoxal/DMSO Adapted from Molecular Cloning (Maniatis), Current Protocols in Molecular Biology, and J. Yost Lab (UMN) Objective: Glyoxal/DMSO gels are great for determining sizes of RNA especially in conjunction
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Incubate at 42 ° for 30-60 min for reverse transcription to proceed. For higher specificity and better resolution of RNA secondary structures, one can use up to 50 °C with Superscript II™ enzyme and up to 60 °C with the Superscript III™ enzyme. DMSO added
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