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- 详细信息
- 文献和实验
- 技术资料
- 库存：
期货
- 规格：
W420761-1ml
中文名：WAY-117345
英文名：WAY-117345
纯度：10mM in DMSO
货号：W420761
Cas号：1164558-25-1
存储温度：-80℃储存
运输条件：特低温运输
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
411400 (Boehringer). For 10mM stock, weigh out 1mg (light sensitive!), add 27ul DMSO to solubilize, then add 12ul 3M NaOAc, pH5, mix, then 275ul H2O, Don''t make up large amountrs as this is not very stable, even at -80C. Diluted luciferin solution
In Situ HYBRIDIZATION TO TISSUE SECTIONS
-Sevag extract (2X) and NaOAc/EtOH ppt. Resuspend in TE @ 1mg/ml. Check on gel for completeness of digestion. Transcription reaction (25ml): 9.5ml DEP-H2O 5.0ml 5X TB 1.0ml linearized DNA template 1.0ml 10mM rATP 1.0ml 10mM rCTP
Easy Way to Clone Genes From a Phage Library
, and then plate out 1, 5 and 25 microliters from this dilution. It is bad form to let phage grow for too long (it leads to mutagenesis). For long-term storage of phage, combine some phage super with 20 percent DMSO, and freeze. Now go back to the other protocols
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