Raphin1 acetate，2242616-04-0，≥

qPCR 实验中遇到这 3 个问题怎么办？

- 120%，且标准曲线 R2≥ 0.99，引物的扩增效率才是合格的，定量出来的 CT值才可以用来计算。且扩增效率越接近 100%，引物的扩增性能越优。 1、若扩增效率不满足 90 - 120%，那 CT值偏大是因为扩增效率不达标导致的，需要重新设计引物。 2、若引物的扩增效率满足 90 - 120% 前提下，CT 值仍然很大，则是基因本身表达量低造成的 CT 值偏大，可以提高模板的添加量，但最根本的方法是改为探针法进行定量。探针法的灵敏度是高于染料法的。

How to isolate mRNA(分离mRNA)

of total RNA. For most kits, total RNA used for mRNA preparation should have a concentration of ≥ 2 μg/μl. Total RNA can be prepared with any of the commonly used protocols. One should keep in mind, though, that RNA prepared with a standard phenol

Crystallization of Kinesin Family Motor Proteins

parameters.Crystals of monomeric rat Kinesin-1Table 1: Crystallization conditions for kinesin motor protein constructsConstructMethodConditionsReferencesHuman Kinesin-1 hK349Sitting drop 4°C5 mg/ml protein in 50 mM Na-acetate, pH 4.6, 75 mM KCl, 3.5% (w/v