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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
WAY-620097
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
731827-03-5
- 规格:
W417666-5mg/W417666-25mg/W417666-100mg
| 规格: | W417666-5mg | 产品价格: | ¥499.9 |
|---|---|---|---|
| 规格: | W417666-25mg | 产品价格: | ¥1799.9 |
| 规格: | W417666-100mg | 产品价格: | ¥4999.9 |
英文名:WAY-620097
纯度或规格:≥95%
SPU:W417666
CAS:731827-03-5
存储温度:-20°C储存
运输条件:超低温运输
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文献和实验Immunofluorescence / Confocal Microscopy Protocol
min 4% paraformaldehyde in 0.1M phosphate buffer pH 7.4, 0.03M sucrose on ice. ** 3. Wash 2x PBS/1%BSA. From now on everything can be at room temp, or on ice. 4. Permeabilize 5 min RT in 0.2% saponin, PBS, 0.03M sucrose, 1% BSA.
In Situ Hybridization Using Digoxigenin Labeled Probes
and the attached fat body. If this does not occur, carefully dissect away the fat body 5) Transfer the "fat free" glands into the fixative and incubate for 4-5 minutes 6) Pick up the coverslip and glands with a clean slide by carefully touching the slide
免疫荧光操作(共聚焦显微镜)-Immunofluorescence Protocol for Confocal Microscopy
tissue culture tubes, not microfuge tubes, and use low speed spins to pellet - just enough to get the cells down in 3-5 min. This way the cell "pellet" is always soft and easy to resuspend with gentle swirling. On chambered coverglass I make sure
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