WAY-638833，794576-60-6，≥95%，阿拉

Tracking individual chromosomes with integrated arrays of Lacop sites and GFP-Laci repressor

for less rapid time-lapse imaging (intervals ≥ 60 seconds) on larger fields and confocal microscopy (see below) for very rapid time-lapse imaging (intervals ≤ 2 seconds) on small regions of interest (typically one yeast nucleus). For very long imaging times

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

. PCR was also used to screen for positive recombinants and to verify correct integration. Primers The short primers (20�30 nt) were purchased from TAG, Copenhagen, Denmark, whereas the longer primers (60�140 nt