RGD peptide (GRGDNP)，114681-65

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

by adding 1µl Rnase A (10mg/ml) and 20µl of 5M NaCl. Incubate the samples in a 65° water bath or heat block for 4-5 hours to overnight (note 5); ;After the reverse cross-linking the elutes are treated with 10µl of 0.5M EDTA, 20µl of 1M Tris-HCL, pH

脂肪组织蛋白质提取方法

in a buffer containing urea (7 M), thiourea (2 M), CHAPS (2% w/v), DTT (65 mM), ampholines (0.5 % v/v), orthovanadate (10mM) and protease inhibitors. Proteins were separated by two-dimensional electrophoresis (2D-PAGE) and visualised by silver staining