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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Chromotrope FB
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
3567-69-9
- 规格:
C121938-50g/C121938-500g/C121938-25g/C121938-1kg/C121938-10g/C121938-100g
| 规格: | C121938-50g | 产品价格: | ¥111.9 |
|---|---|---|---|
| 规格: | C121938-500g | 产品价格: | ¥691.9 |
| 规格: | C121938-25g | 产品价格: | ¥61.9 |
| 规格: | C121938-1kg | 产品价格: | ¥1258.9 |
| 规格: | C121938-10g | 产品价格: | ¥34.9 |
| 规格: | C121938-100g | 产品价格: | ¥196.9 |
英文名:Chromotrope FB
纯度或规格:Dye content,50%
SPU:C121938
CAS:3567-69-9
存储温度:-
运输条件:常规运输
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文献和实验cycle and in a poor yield of PCR product. The initial denaturation should be performed over an interval of 1-3min at 95°C if the GC content is 50% or less. This interval should be extended up to 10min for GC-rich templates. If the initial denaturation
is any nucleotide, R is purine (A, G) and Y is pyrimidine (C, U). Avoid targeting introns, since RNAi only works in the cytoplasm and not within the nucleus. Avoid sequences with > 50% G+C content. Avoid stretches of 4 or more nucleotide repeats
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
of amplification, as the mean length of target DNA is decreased: for templates of 300bp or less, denaturation temperature may be reduced to as low as 88℃ for 50% (G+C) templates (Yap and McGee, 1991), which means one may do as many as 40 cycles
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