Tris-NTA，862778-60-7，≥98%，阿拉丁

Raf 蛋白 Ras 结合结构域的简并进化库合成以及利用片段互补法快速筛选二氢叶酸还原酶的快速折叠且稳定的克隆

. 洗脱后在样品中加入 DDT 是因为 Ni-NTA 不推荐使用 DDT。可以再 NaOH 或其他合适弱碱，如 Tris 或 NaOAc，调节洗出液 pH。 28. 如果样品不是立即用于性质鉴定，我们建议在样品中加入 40% ( V/V ) 的甘油和 1 mmol/L NaN3，-20℃ 保存。在性质鉴定前通过透析或超滤去除甘油。或者，不加甘油，用液氮速冻样品，-80°C 保存。 29. 根据 QIAEXpressionnist Handbook，用 TFB 缓冲液（RbCl ) 制作

Assisted large fragment insertion by Red/ET-recombination (ALFIRE)―an alternative and enhanced method for large fragment recombineering

, and a final elongation of 5 min at 72°C. An aliquot of each PCR product was analysed by gel electrophoresis. The rest of the PCR product was ethanol-precipitated, resuspended in Tris�HCl (10 mM pH 8.5) and used for transformation into electrocompetent bacteria

Disulfide Conjugation of Peptides to Oligonucleotides and Their Analogs

perchlorate (AnalaR, BDH Chemicals) 2.0 M Tris·Cl, pH 6.8 Formamide (p.a. ≥99.0%, Fluka