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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
Vitamin D₂ solution
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- 规格:
V466257-1ml
英文名:Vitamin D₂ solution
纯度或规格:≥98%, 100μg/mL in ethanol
SPU:V466257
CAS:-
存储温度:-20°C储存
运输条件:超低温运输
查看阿拉丁官网此产品相关对应页面:
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文献和实验Concentration of DNA by ethanol precipitation
for at least 30 min, or at -20℃ overnight. or alternatively 1. Combine 95 ml of 100% ethanol with 4 ml of 3 M NaOAc (pH 4.8) and 1ml of sterile water. Mix by inversion and store at -20℃. 2. Add 2.5 volumes of cold ethanol/acetate solution to the nucleic acid
Concentration of DNA by ethanol precipitation
, 3. Place at -70°C for at least 30 min, or at -20°C overnight. or alternatively 1. Combine 95 ml of 100% ethanol with 4 ml of 3 M NaOAc (pH 4.8) and 1ml of sterile water. Mix by inversion and store at -20°C. 2. Add 2.5 volumes
Transformation of E. coli by Electroporation
absolute ethanol. Ice 15 min. 3. Centrifuge at >12,000 x g for 15 min at 4° C. Carefully decant the supernatant. 4. Wash the pellet with 1 ml of 70% ethanol. Centrifuge at >12,000 x g for 15min at room temperature. Remove the supernate. 5. Air
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