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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
NHS-PEG4-(m-PEG12)3-ester
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
1334178-03-8
- 规格:
N596237-500mg/N596237-250mg/N596237-100mg
| 规格: | N596237-500mg | 产品价格: | ¥26880.9 |
|---|---|---|---|
| 规格: | N596237-250mg | 产品价格: | ¥13440.9 |
| 规格: | N596237-100mg | 产品价格: | ¥7980.9 |
英文名:NHS-PEG4-(m-PEG12)3-ester
纯度或规格:≥90%
SPU:N596237
CAS:1334178-03-8
存储温度:-20°C储存
运输条件:超低温运输
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文献和实验Coupling Peptides To Resin For Affinity Purification
5% ethylene diamine in water. Incubate 15' at RT. Wash with 10 volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate
Coupling Peptides To Resin For Affinity Purification
volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add
Revised amino-allyl labeling protocol
, all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cy-dyes from coupling to free amine groups in solution. 3. Add 450 m L water to each reaction. C. Cleanup Add 500 m L neutralized, diluted reaction
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