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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Polycarbonate
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
25037-45-0
- 规格:
P301656-250g/P301656-1kg
| 规格: | P301656-250g | 产品价格: | ¥823.9 |
|---|---|---|---|
| 规格: | P301656-1kg | 产品价格: | ¥2038.9 |
英文名:Polycarbonate
纯度或规格:melt index:10 g/10 min (300°C/1.2kg)
SPU:P301656
CAS:25037-45-0
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文献和实验TOP10 chemically competent cells
Centrifuge at 3000g at 4°C for 10 minutes in a flat bottom centrifuge bottle. Flat bottom centrifuge tubes make the fragile cells much easier to resuspend It is often easier to resuspend pellets by mixing before adding large
Construction and Characterization of Adenovirus Vectors
stock solution with H2 O and sterilize by autoclaving for 45 min at 121°C. Complete medium Dialysis buffer (10 mM Tris-HCl, pH 8.0) Sterilize by filtration through a 0.2-µm filter. DNase I (Ad) Ethidium bromide
lysis buffer (high salt) ) 2 x 1 ml CHIP wash buffer 2 x 1 ml TE elute immunoprecipitations: add 75 µl elution buffer incubate for 10 min at 65 °C spin, take supernatant, elute pellet again with 75 µl
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