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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
避光;-20°C储存;充氩
- 英文名:
m-PEG-NHS ester
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
92451-01-9
- 规格:
M658882-250mg/M658882-1g/M658882-100mg
| 规格: | M658882-250mg | 产品价格: | ¥1099.9 |
|---|---|---|---|
| 规格: | M658882-1g | 产品价格: | ¥2599.9 |
| 规格: | M658882-100mg | 产品价格: | ¥729.9 |
英文名:m-PEG-NHS ester
纯度或规格:MW5000
SPU:M658882
CAS:92451-01-9
存储温度:避光;-20°C储存;充氩
运输条件:超低温运输
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文献和实验Crystallization of Kinesin Family Motor Proteins
4, pH 6.8, 6.8% PEG 8000, 1 M NaCl, 2 mM ATP, 3.5 mM DTT, 10 mM MgCl2Reservoir: 25 mM Na2PO4 pH 6.8, 13.5% PEG 8000, 2 M NaCl, 7 mM DTTKozielski et al., 1999Eg5 1-368HsKSPSitting Drop 4°C5 mg/ml protein in 9% PEG-3350, 50 mM PIPES pH 6.8, 100 mM NaNO
Coupling Peptides To Resin For Affinity Purification
5% ethylene diamine in water. Incubate 15' at RT. Wash with 10 volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate
Coupling Peptides To Resin For Affinity Purification
volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add
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