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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存;低温运输
- 英文名:
4,4'-Azobis(4-cyanovaleric acid)(ACVA)
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
2638-94-0
- 规格:
A106307-100g/A106307-25g/A106307-5g/A106307-500g
| 规格: | A106307-100g | 产品价格: | ¥209.90 |
|---|---|---|---|
| 规格: | A106307-25g | 产品价格: | ¥59.90 |
| 规格: | A106307-5g | 产品价格: | ¥39.90 |
| 规格: | A106307-500g | 产品价格: | ¥999.90 |
中文名:4,4'-偶氮双(4-氰基戊酸)(ACVA)
英文名:4,4'-Azobis(4-cyanovaleric acid)(ACVA)
应用:聚合物合成自由基引发剂。
纯度:≥98%,20% Water
货号:A106307
Cas号:2638-94-0
存储温度:2-8°C储存
运输条件:低温运输
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文献和实验General Protocol to Prepare Neuronal Cultures
. 12. Count cells with a hemocytometer. 13. Further dilute the cells with B27/Neurobasal/0.5 mM glutamine to the desired plating density. We recommend 32 x 103 cells/2 cm2 of substrate in 0.4 ml/2 cm2 substrate. 14. Incubate the cells at 37ºC
Native Chromatin Preparation and Illumina/Solexa Library Construction
is empty. Collect the entire effluent in the same tube (from Step 18). 20. Count cells and check CD4+ T cell purity. Recovery is typically ~20%. Purified CD4+ T cells with ~ 95%-98% purity are used for native chromatin preparation
Preparation of Poly A+ RNA and Northern Analysis
sperm DNA. Prehybridization SolÕn Hybridization SolÕn ddH2O 15.3 ml ddH2O 15.3 ml Formamide 15 ml Formamide 15 ml 20 x SSPE 12.5 ml 20 x SSPE 12.5 ml 20% SDS 0.25 ml 20% SDS 0.25 ml 50 x DenhardtÕs 5 ml 50 x DenhardtÕs 5 ml Salmon Sp. DNA 2 ml Salmon
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