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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存
- 英文名:
4,4'-Azobis(4-cyanovaleric acid)
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
2638-94-0
- 规格:
A106307-5g/A106307-500g/A106307-25g/A106307-100g
| 规格: | A106307-5g | 产品价格: | ¥39.9 |
|---|---|---|---|
| 规格: | A106307-500g | 产品价格: | ¥999.9 |
| 规格: | A106307-25g | 产品价格: | ¥59.9 |
| 规格: | A106307-100g | 产品价格: | ¥209.9 |
英文名:4,4'-Azobis(4-cyanovaleric acid)(ACVA)
纯度或规格:≥98%, 20% Water
SPU:A106307
CAS:2638-94-0
存储温度:2-8°C储存
运输条件:低温运输
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文献和实验; pH = 8.0 (store at 4o C). Conc. Stock %(w/w) 50 ml 100 ml 150 ml 250 ml EDTA 0.5 M 372.3g/M 16.98 9.31g 18.62g 27.92g 46.55g NaOH ~0.5 M 40g/M 1.82 1.014g 2.028g 3.042g
General Protocol to Prepare Neuronal Cultures
. 12. Count cells with a hemocytometer. 13. Further dilute the cells with B27/Neurobasal/0.5 mM glutamine to the desired plating density. We recommend 32 x 103 cells/2 cm2 of substrate in 0.4 ml/2 cm2 substrate. 14. Incubate the cells at 37ºC
Native Chromatin Preparation and Illumina/Solexa Library Construction
is empty. Collect the entire effluent in the same tube (from Step 18). 20. Count cells and check CD4+ T cell purity. Recovery is typically ~20%. Purified CD4+ T cells with ~ 95%-98% purity are used for native chromatin preparation
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