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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Phosalone solution
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
2310-17-0
- 规格:
P114591-2ml/P114591-1ml
| 规格: | P114591-2ml | 产品价格: | ¥190.9 |
|---|---|---|---|
| 规格: | P114591-1ml | 产品价格: | ¥119.9 |
校准仪器和装置;评价方法;工作标准;质量保证/质量控制;其他。
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文献和实验Yeast IF with Methanol/Acetone Dehydration
containing 1.2M sorbitol. Be very gentle with the cells! Resuspend in 0.5 mls sorbitol buffer. The cells can be stored at this point overnight at 4¡C. Zymolyase treat the cells with 30 ul 10 mg/ml Zymolyase (100T) at 30¡C for anywhere between 10-30 min
FIXATION WITH FORMALDEHYDE-ACETONE
), in a bottle designated for fixatives. Cap tightly and warm up in a water bath. 5. Acetone, chilled to -20o C in a freezer. Transfer into 100 ml beakers designated for fixation, each coverslip needs a separate beaker. 6. Forceps for fixation.
Yeast IF with Methanol/Acetone Dehydration
in 0.5 mls sorbitol buffer. The cells can be stored at this point overnight at 4¡C. Zymolyase treat the cells with 30 ul 10 mg/ml Zymolyase (100T) at 30¡C for anywhere between 10-30 min or more. Examine cells on a phase microscope. When cells are dark
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