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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°C储存
- 英文名:
Carbendazim solution
- 库存:
现货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
10605-21-7
- 规格:
C102517-1ml
英文名:Carbendazim solution
纯度或规格:分析标准品, 100ug/ml,u=3% in ethanol
SPU:C102517
CAS:10605-21-7
存储温度:-20°C储存
运输条件:超低温运输
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文献和实验Concentration of DNA by ethanol precipitation
. 100 ug or more) into several small (0.5 ml) microcentrifuge tubes for frozen storage because repeated freezing and thawing is not advisable. Notes on precipitation of nucleic acids A. General rules Most nucleic acids may be precipitated by addition
Concentration of DNA by ethanol precipitation
, 3. Place at -70°C for at least 30 min, or at -20°C overnight. or alternatively 1. Combine 95 ml of 100% ethanol with 4 ml of 3 M NaOAc (pH 4.8) and 1ml of sterile water. Mix by inversion and store at -20°C. 2. Add 2.5 volumes
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
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