1-tert-butoxycarbonylamino-4,4

In Situ Hybridization Using Digoxigenin Labeled Probes

siliconized slide place two drops of 45% acetic acid and a clean siliconized 18 mm square coverslip. On the coverslip place a small drop (2-3 mm) of fixative( 1/2/3 mixture of lactic acid/water/glacial acetic acid) 3) In the first drop of 45% acetic

【资源】蛋白质组学指南-来自杜克大学（Molecular Biologist’s Guide to Proteomics）

.............................................................................................50 Amino acid sequence database searching .........................................................................................................50 De novo peptide sequence information

Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOF MS Analysis

from gels. Optimized protein recoveries were determined to be 89% for BSA and 58% for Phos B, supporting the hypothesis of an inverse relationship between recovery and protein size. The traditional practice of “fixing gels” using methanol and acetic acid