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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgG2a, kappa
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
单克隆
- 保质期:
12 months
- 级别:
一抗
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Mouse
- 应用范围:
IHC, WB
- 抗体英文名:
Anti-Kir2.4 K+ channel Antibody (N465/11)
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1188.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥1968.0 |
| 规格: | 1mg | 产品价格: | ¥9840.0 |
| 产品名 | Anti-Kir2.4 K+ channel Antibody (N465/11) |
| 货号 | RHN26801 |
| 靶标 | IRK-4, Inward rectifier K(+) channel Kir2.4, KCNJ14, Potassium channel, inwardly rectifying subfamily J member 14, IRK4, ATP-sensitive inward rectifier potassium channel 14 |
| 克隆号 | N465/11 |
| 克隆类型 | Monoclonal |
| 同种型 | IgG2a, kappa |
| 宿主 | Mouse |
| 应用 | IHC, WB |
| 纯度 | >95% as determined by SDS-PAGE. |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 说明 | For research use only. |

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文献和实验Determination of Phosphoinositide Binding to K+ Channel Subunits Using a ProteinLipid Overlay Assay
, we describe a rapid and convenient biochemical assay that can be used to show direct binding of potassium channel subunits to anionic phospholipids. This method has been used to demonstrate the differences in affinity between members of the Kir3.0 family
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
Protocol for anti-HA antibody Western Blotting
room. Expose film for ~2 mins. Notes on the primary antibody: Source - Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in ―20c
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