4-chloro-α-Cyanocinnamic Acid，

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The pSC101BADγβαA-I-SceI[amp] vector was assembled by PCR amplification of the I-SceI gene together with the Tet-Repressor, Tet-Promoter and ampicillin resistance gene sequences from the PsiI-digested pST98AS plasmid (AF170483 ) (16) [oligo

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of Uracil and Cytosine Pyrimidine Bases   Materials Sodium hydroxide (NaOH), ≥98%

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) Trimethyl orthoformate, 98% pure (Aldrich) p ‐Toluenesulfonic acid monohydrate, ≥98.5% pure (Aldrich)