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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8°C储存;低温运输
- 英文名:
(1S,4S)-2-methyl-2,5-diazabicyclo[2.2.1]heptane dihydrochloride
- 库存:
期货
- 供应商:
上海阿拉丁生化科技股份有限公司
- CAS号:
127420-27-3
- 规格:
M637538-1g
中文名:(1S,4S)-2-methyl-2,5-diazabicyclo[2.2.1]heptane dihydrochloride
英文名:(1S,4S)-2-methyl-2,5-diazabicyclo[2.2.1]heptane dihydrochloride
纯度:≥97%
货号:M637538
Cas号:127420-27-3
存储温度:2-8°C储存
运输条件:低温运输
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文献和实验PBS 0.3% triton X100 0.5% BSA 10X PBS (g/l) 200 NaCl 5 KCl 5 KH2PO4 27.8 Na2HPO4.2H2O EBR (Ringer's Solution) Am. Nat. 70, 218-225. Modified to use HEPES buffer instead of Tris.
Single and Double FISH protocols for Drosophila
stock solution; Jackson ImmunoResearch Laboratories Inc., West Grove. PA, USA.; Cat No. 713-166-147]. 8. Embryo mountant: 70% glycerol, 2% DABCO (1,4-Diazabicyclo [2.2.2.] Octane; Sigma, USA; Cat No. D-2522). 9. Microscope slides. 10
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBRYOS
on a Millipore device with a nitex filter; rinse well with 0.1% Triton X-100 from a squirt bottle. Transfer embryos to 3:1 heptane:fix with a paint brush and fix for 20-40 min. Fix in a glass scintillation vial using 6.1ml HEPES Fix Mix + 0.75ml 37
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