- ￥7956
- Biorbyt
- orb2276194
- 英国
- 2026年08月07日
- ICC, IHC, IHC-P, IP, WB
- Mouse
- Bovine, Human, Porcine, Rabbit, Sheep
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- 文献和实验
- 技术资料
- 抗体名：
KRT8 / CK8 / Cytokeratin 8 Antibody (clone C-43) 抗体
- 抗体英文名：
KRT8 / CK8 / Cytokeratin 8 Antibody (clone C-43)
- 浓度：
1 mg/ml
- 应用范围：
ICC, IHC, IHC-P, IP, WB
- 宿主：
Mouse
- 适应物种：
Bovine, Human, Porcine, Rabbit, Sheep
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb2276194
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
IgG1
- 免疫原：
详询
- 规格：
0.05 mg
描述: KRT8 / CK8 / Cytokeratin 8 Antibody (clone C-43)
克隆号: C-43
别名: KRT8, CARD2, CK-8, CYK8, Cytokeratin-8, CK8, K2C8, K8, KO, Keratin-8, Type-II keratin Kb8, Cytokeratin 8, Keratin 8
应用稀释比例: ICC, IHC, IHC-P (20 µg/ml), IP, WB
应用说明: Does not react with: Xenopus, Chicken, Rat, Hamster, Mouse
免疫原: Cytoskeleton preparation from HeLa human cervix carcinoma cell line
防腐剂: PBS, pH 7.4, 15 mM Sodium Azide
纯化方法: Purified by protein-A affinity chromatography. Greater than 95% by SDS-PAGE.
特异性: The antibody C-43 reacts with Cytokeratin 8 (52.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验Staining protocols for placental explant cultures
there is less likelihood of damaging the culture during processing. Incubate overnight (at least) at 4°C in blocking solution (4% BSA/PBS). Include 0.02% sodium azide if the plates are to be kept longer. Incubate in 1st antibody for 30 min at room temperature. Ideally
Immuno-Laser Capture Microdissection
during LCM without coverslip. The smaller spot size allows avoidance of interspersed T cells. Magnification x100 (C) and x400 (D). Fig. E: Lymph node stained for the proliferation-associated antigen Ki67, using MIB1 antibody (dilution, 1:10
Staining protocols for placental explant cultures
over the explant, preventing it from drying out. Antibody should be diluted in 4% BSA/PBS. A good positive control antibody is anti-cytokeratin 7 (OV-TL 12/30; Dako; use at 1/50). This identifies the outgrowing cells unequivocally as trophoblast. Anticytokeratin
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