Anti-Human CD62E (E-Selectin)

FITC荧光二抗？――EarthOx新型DyLight 488绿色荧光

的价格比常规二抗 只高20%左右，但是Dylight的效价通常是常规荧光二抗的2-4倍左右，因此就最终的性价比，Dylight更胜一筹。举例来说，EarthOx的 FITC标记的山羊抗小鼠IgG二抗（货号为E031210），100μl和500μl报价分别是￥160和￥640；而相应的EarthOx的 Dylight 488标记的山羊抗小鼠IgG二抗（货号为E032210），100μl和500μl报价分别是￥200和￥800，但是在具体使用时，比如免疫荧光实验，其推荐稀释比率分别为1:100

Dual- and Triple-Color Fluorospot

is the availability of additional amplification systems compatible with, e.g., FITC/anti-FITC and biotin/avidin and, in particular, automated readers designed for the analysis of three-colored spots. One amplification strategy that has been evaluated is to use

Flow Cytometry: Immunofluorescence Staining of Activated and Resting Human Platelets

for 30 minutes at room temperature.6. Wash two times in PBS and resuspend cell pellet in PBS. Cells may be stored at 4℃ for up to 8 hours.7. Stain activated platelets by direct immunofluorescence using CD62P-FITC, catalog number 555523 (31794×), at 20 μL