Anti-Mouse CD19 - Purified (Ph

Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells

volume of Dynabeads taken  from the vial (step 2). 2.        Activation of Mouse T Cells 1)        Start with 8 × 104 purified T cells in 100-200 µl medium in a 96-well tissue culture plate. 2)        Add 2 µl Dynabeads Mouse T-Activator CD3/CD

Expression and Preparation of Fusion Proteins from Recombinant gt1.1 Phages

-level expression, the relative stability of β-galactosidase fusion proteins, and simple approaches to purify the fusion proteins. After the desired clone is detected and purified, it is often necessary to obtain preparative amounts of recombinant protein

BAC Transgenes, DNA Purification, and Transgenic Mouse Production

expression occurs at physiological levels in the appropriate cell types while recapitulating normal developmental time frames. Genomic libraries prepared in bacterial artificial chromosomes (BACs) for the mouse and human genome sequencing projects are a ready