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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
2-8℃
- 适应物种:
Human/Mouse
- 供应商:
朗智(上海)生物科技有限公司
- 规格:
25 µg
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文献和实验3 mAb,PE-Cy5 50T/100T 1200/1800 L0005 Mouse anti-Human CD3 mAb,APC 50T/100T 1000/1500 L0006 Mouse anti-Human CD3 mAb,Biotin 50T/100T
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
. 36. Resuspend the cells in OP9 medium, seed 5 x 105 cells per 10-cm dish containing ~80% confluent OP9 cells in 10 mL of OP9 medium, and add 5 ng/mL of recombinant human Flt-3L. Return the dish to the incubator. The number of 10-cm dishes
Cell Surface Immunofluorescence Staining Protocol
-CD4-Biotin, and anti-CD8-APC) and incubate on ice for 15-20 minutes in the dark. 9) Wash 2X with at least 2 ml of Cell Staining Buffer by centrifugation at 350 x g for 5 minutes. 1) If using a purified primary antibody, resuspend
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