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- 详细信息
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- 文献和实验
- 技术资料
- 英文名：
ECL Western Blotting Substrate
- 供应商：
普洛麦格
- 规格：
250ml
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文献和实验Western blotting using ECL reagent
REAGENTSECL Western blotting kit (Amersham Life Science; cat# RPN2108): contains second antibodies for both mouse and rabbit, substrate and milk blocker (the milk blocker is not normally used when using ruminant samples).Hybond ECL nitrocellulose
(All steps with agitation, unless done O/N at 4℃)►Rinse membrane once in TBS-T►Block the membrane with TBS-T/ 5% milk (or HIHS) for 30 min room►temp (can be left at 4℃ O/N here)►Rinse membrane briefly 2X with TBS-T and then once for 10 min with TBS
SDS-PAGE Western Blotting Protocols
liquid. 11. Pour stacking gel to fill the remaining space between the glass plates. 12. Insert comb and let polymerize (this takes ~10-15’). 13. Prepare 20-30ml of sample, as follows: 14. Boil samples for at least 3’. 15. Place gel
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