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- promega
- V3941
- 中国
- 2026年02月04日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商：
普洛麦格
- 英文名：
X-Gal
- 规格：
100mg
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文献和实验RNA Purification Protocol for 100-200 mg of Animal Tissue
. Storage at -70°C. Fresh tissue can also be used. Work quickly and keep the sample on ice at all times. 2. Add 100-200 mg frozen ground tissue or fresh tissue to a 15 mL centrifuge tube containing 6 ml RCL Buffer. Homogenize the sample using 5-10
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
reaction buffer can be stored for at least one year at room temperature in foil-covered container. X-gal stock(40X) 40 mg/ml X-gal in dimethylformamide Store at -200 C in foil covered glass container. Rinse many times in PBS
ml of X-gal staining buffer with 1 mg/ml X-gal (make 40mg/ml stock in DMF, store at -20OC; don't use if discolored). Incubate at 30OC for 24 -48 hrs, or less if expression is strong 5. Clear in acetone, rehydrate and stain in carmine alum O/N.
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