- 询价
- promega
- E6079
- 中国
- 2025年12月22日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
普洛麦格
- 英文名：
Absorbance Filter Paddle, 750nm
- 规格：
1 each
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文献和实验Measure the optical absorbance at 230 nm (UV). The absorbance of a 1 mg/ml concentration of pea bud histone at 230 nm equals 3.5. OD units. The absorbance follows the Beer-Lambert law, and is linear with histone concentration. Since it is merely
or gamma globulin are recommended)in 100 µl volume.See how to set up an assay for suggestions as to setting up the standards.5.Add 5 ml dye reagent and incubate 5 min.6.Measure the absorbance at 595 nm.AnalysisPrepare a standard curve of absorbance
Use of the Bradford Protein Assay in a Microtiter Plate Format
well. Any multi-well microtiter plate reader equipped with a 595 nm filter. Procedure Note: do all determinations in duplicate or triplicate 1. Pipette 0, 2, 4, 6, 10, 15 and 20 µl of BSA (1 mg/ml) into assigned wells of a 96-well plate
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