重组人SFTPB蛋白(N-His标签)

名称：重组人SFTPB蛋白(N-His标签)

规格：100μg

纯度：>90% as determined by SDS-PAGE.

用途：SDS-PAGE,WB,ELISA,Immunogen

注意：由于产品数量众多，具体详细产品说明书欢迎免费索取。本公司产品仅供科研使用，不用于临床或其他诊断治疗用途。

在重组蛋白生产中应用广泛。蛋白标签是一种方便有效的工具，可以提高重组蛋白的溶解性和稳定性；便于目的蛋白的检测、示踪和纯化。随着研究的需求和技术的发展，各种标签应运而生，多样化的标签可以满足不同的应用场景。许多重组蛋白在生产过程中溶解度较差，可以使用融合标签以增加溶解度，融合标签可用于目的蛋白没有特异性抗体的多种应用，如Western Blot、免疫沉淀、ELISA等。

蛋白标签类型主要分为三类，适用于不同的应用场景：表位标签、亲和标签和荧光标签。

- 表位标签往往是短肽序列，可用于免疫学应用，如 Western Blot 和免疫共沉淀。最常用的表位标签有His、FLAG、HA等。
- 亲和标签一般较长，可增加蛋白溶解度，广泛应用于重组蛋白的纯化，如SUMO、Trx、MBP等。
- 荧光标签可用于活细胞和死细胞检测，最常用的荧光蛋白包括绿色荧光蛋白（GFP）、橙色荧光蛋白（OFP）、红色荧光蛋白（RFP）和黄色荧光蛋白（YFP）。它们被广泛用于影像学研究，如细胞定位和共表达实验。

重组人SFTPB蛋白(N-His标签)如何选购？

考虑融合标签的影响。任何一类标签处于氨基酸序列的任一位置，都具有影响目的蛋白表达或功能的可能性。主要原因是标签可能会干扰蛋白的正确折叠，致使目的蛋白失活或形成包涵体。其次，标签可能会中断亚细胞定位信号，这种情况下，蛋白能够正确翻译和折叠，但在细胞内所处的位置是错误的。因此，您需要知道添加的标签对目的蛋白的表达是否有影响。

考虑是在N-端还是C-端标记。N-端或C-端标记的选择还需要根据蛋白结构、定位等特性。然而，倘若你没有确切的蛋白结构，或蛋白功能域图谱，建议分别构建N-端标记和C-端标记的表达克隆，以检测哪个更有效。

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PAB00140    兔抗DDX58多克隆抗体(WB,IHC,ELISA)    DDX58 Rabbit Polyclonal Antibody(Human)
PAB00141    兔抗AIFM1/AIF多克隆抗体(WB,IHC,ELISA)    AIFM1/AIF Rabbit Polyclonal Antibody(Human)
PAB00142    兔抗CLDN1多克隆抗体(WB,IHC,ELISA)    CLDN1 Rabbit Polyclonal Antibody(Human)
PAB00143    兔抗ANGPTL1多克隆抗体(WB,IHC,ELISA)    ANGPTL1 Rabbit Polyclonal Antibody(Human)
PAB00144    兔抗SNAI1多克隆抗体(WB,IHC,ELISA)    SNAI1 Rabbit Polyclonal Antibody(Human)
PAB00145    兔抗DDAH2多克隆抗体(WB,IHC,ELISA)    DDAH2 Rabbit Polyclonal Antibody(Human)
PAB00146    兔抗EFEMP2多克隆抗体(WB,IHC,ELISA)    EFEMP2 Rabbit Polyclonal Antibody(Human)
PAB00147    兔抗CD160多克隆抗体(WB,IHC,ELISA)    CD160 Rabbit Polyclonal Antibody(Human)
PAB00148    兔抗BMP15多克隆抗体(WB,IHC,ELISA)    BMP15 Rabbit Polyclonal Antibody(Human)
PAB00149    兔抗AGR2多克隆抗体(WB,IHC,ELISA)    AGR2 Rabbit Polyclonal Antibody(Human)
PAB00150    兔抗IL18BP多克隆抗体(WB,IHC,ELISA)    IL18BP Rabbit Polyclonal Antibody(Human)
PAB00151    兔抗TOMM40多克隆抗体(WB,IHC,ELISA)    TOMM40 Rabbit Polyclonal Antibody(Human)