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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
蛋白表达区间:Thr678-Arg751
- 保质期:
See instructions
- 英文名:
标签:Tag free
- 库存:
表达系统:E.coli
- 供应商:
上海经科化学科技有限公司
- 规格:
10ug/50ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥840.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥2520.0 |
| 规格: | 500ug | 产品价格: | ¥12320.0 |
| 规格: | 1mg | 产品价格: | ¥17600.0 |
- Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
- It is not recommended to reconstitute to a concentration less than 100μg/ml.
- Dissolve the lyophilized protein in distilled water.
- Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
- The product is shipped at ambient temperature.
- Upon receipt, store it immediately at the temperature listed below.
- Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
- Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
- Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
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文献和实验Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 α-chain. Human C5a has four α-helices plus three intrachain disulfide bonds that create a triple loop structure. Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 小鼠补体C5a (C5a)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗小鼠 C5a 单抗包被于酶标板上,标准品和样品中的 C5a与单抗结合,加入生物素化的抗小鼠 C5a ,形成
人补体片段C5a (C5a)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 C5a 单抗包被于酶标板上,标准品和样品中的 C5a与单抗结合,加入生物素化的抗人 C5a ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止液硫酸,在 450
一、实验原理 将抗C5a单克隆抗体包被固相聚苯乙烯板,加入经聚乙二醇(PEG)处理的待测样本,然后依次加入兔抗人C5a-IgC多克隆抗体和过氧化物酶标记的猪抗兔IsC,再加入底物2,2’-连氮-双(3-乙基苯骈唑啉-6-磺酸盐)(ABTS)和H2O2显色,于酶标仪405 mn处测定每分钟光密度增加量(OD/min),以OD/min为纵坐标,C5a标准晶浓度为横坐标绘制标准曲线图,求出待测样本中C5a的含量。 二、实验试剂 1. PBSPH7.2
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