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Human FGF-4(CR08)

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  • ¥840 - 14400
  • SinoBio
  • 国产
  • CR08
  • 2026年04月27日
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    • 文献和实验
    • 技术资料
    • 保存条件

      蛋白表达区间:Ser54-Leu206

    • 保质期

      See instructions

    • 英文名

      标签:Tag free

    • 库存

      表达系统:E.coli

    • 供应商

      上海经科化学科技有限公司

    • 规格

      10ug/50ug/500ug/1mg

    规格:10ug产品价格:¥840.0
    规格:50ug产品价格:¥2520.0
    规格:500ug产品价格:¥10080.0
    规格:1mg产品价格:¥14400.0
    产品描述
    Recombinant Human Fibroblast Growth Factor 4 is produced by our E.coli expression system and the target gene encoding Ser54-Leu206 is expressed.
     
    蛋白编号
    P08620
     
    产品别称
    Fibroblast growth factor 4; FGF-4; Heparin secretory-transforming protein 1; HST; HST-1; HSTF-1; Heparin-binding growth factor 4; HBGF-4; Transforming protein KS3; FGF4; HST; HSTF1; KS3
     
    分子量
    16.9 KDa
     
    表观分子量
    16 KDa, reducing conditions
     
    剂型描述
    Lyophilized from a 0.2 μm filtered solution of PBS, 500mM NaCl, pH7.4.
     
    内毒素
    < 0.01 EU/µg as determined by LAL test.
     
    纯度-SDS-PAGE
    Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
     
    生物活性-Cell Based Assay
    Measured in a cell proliferation assay using MCF7 Human breast cancer cells The ED50 for this effect is 2-20 ng/ml. (QC verified)
    Human intestinal organoids (ipsc source) were cultured with Activin A(Cat#C687) , BMP-4(sample) , EGF (Cat#C029) , Wnt3a (Cat#C22R), Noggin (Cat#C028), R-spondin 1 (Cat#CX83) and FGF-4 (Cat#CR08) . The organoids showed good morphology.
     
    复溶
    • Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
    • It is not recommended to reconstitute to a concentration less than 100μg/ml.
    • Dissolve the lyophilized protein in distilled water.
    • Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
     
    运输条件
    • The product is shipped at ambient temperature.
    • Upon receipt, store it immediately at the temperature listed below.
     
    保存条件
    • Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
    • Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
    • Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
     
    产品背景
    Fibroblast growth factor 4(FGF-4) is a heparin binding member of the FGF family. The human FGF4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus. Mature human FGF4 shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell selfrenewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease.

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    图标文献和实验
    该产品被引用文献

    Fibroblast growth factor 4(FGF-4) is a heparin binding member of the FGF family. The human FGF4 cDNA encodes 206 amino acids (aa) with a 33 aa signal sequence and a 173 aa mature protein with an FGF homology domain that contains a heparin binding region near the C-terminus. Mature human FGF4 shares 91%, 82%, 94% and 91% aa identity with mouse, rat, canine and bovine FGF4, respectively. Human FGF-4 has been shown to exhibit cross species activity. Expression of FGF-4 and its receptors, FGF R1c, 2c, 3c and 4, is spatially and temporally regulated during embryonic development. FGF-4 is proposed to play a physiologically relevant role in human embryonic stem cell selfrenewal. It promotes stem cell proliferation, but may also aid differentiation depending on context and concentration, and is often included in embryonic stem cell media in vitro. FGF-4 is mitogenic for fibroblasts and endothelial cells in vitro and has autocrine transforming potential. It is a potent angiogenesis promoter in vivo and has been investigated as therapy for coronary artery disease.

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    文献支持
    Human FGF-4(CR08)
    ¥840 - 14400