Big Endothelin ELISA/大内皮素ELISA

Big Endothelin-1 (BigET-1) is a peptide of 38 amino acids and is the precursor of Endothelin-1 (ET-1), consisting of amino acids 1-21. ET-1 is a potent vasoconstrictor and is produced by vascular endothelial cells. Accordingly, it has a wide tissue distribution. The cleavage of BigET-1 by the Endothelin-converting enzyme (ECE) converts BigET-1 to Endothelin (ET) and to a C‑terminal fragment. The half-life of ET-1 (1-21) in plasma is less than one minute, which may preclude its use as a diagnostic marker, whereas clearance of the precursor peptide BigET-1 is much slower. As Big-Endothelin-1 is produced at amounts equimolar to ET-1 and has higher plasma stability, it is more suitable for diagnostics and monitoring of heart failure (Gergei et al., 2018). Hence, Big ET-1 has the same physiological role as ET-1 while being a more reliable marker. The Big Endothelin-1 immunoassay is a 5.5 hour, 96-well sandwich ELISA for the quantitative determination of Big Endothelin-1 in serum and plasma. The assay employs human serum-based standards to ensure the measurement of biologically reliable data.