Recombinant T7 RNA Polymerase

T7 RNAP.

T7 RNA polymerase active enzyme synthesizes RNA at an increasing degree of that of E. coli RNA polymerase and it terminates transcription often. T7 RNA polymerase is very selective for initiation at its own promoter sequences and is resistant to antibiotics that inhibit E. coli RNA polymerase. In-vitro transcription of mRNA is achieved via bacteriophage T7 RNA polymerase using its ability to produce full-length RNA transcripts with high reliability, thoughT7 RNAP can manufacture as well immunostimulatory by products for example dsRNA which affect protein expression.

T7 RNA polymerase Recombinant protein is produced by bacteriophage T7 DNA which is expressed in recombinant E. coli bacterial system

T7 RNA Polymerase is a DNA-dependent 5'→ 3' RNA polymerase which specifically recognizes T7 promoter sequences.

T7 Bacteriophage RNA Polymerase gene

Sterile Filtered clear solution.

(500U/ul) in 50mM Tris-HCl (25°C, pH 7.9), 0.1M NaCl, 0.1mM EDTA, 2mM DTT, 0.1% Triton X-100 and 50% glycerol.

Transcription Buffer 40mM Tris-HCl (25°C, pH-8), 20mM MgCl₂, 2.5mM TCEP & 2mM spermidine.

Two years when stored at -20°C, 2 weeks at 4°C.  DO NOT STORE AT -70C.

1U is defined as the amount of enzyme required to incorporate 1nmol of [³H] ATP into acid-insoluble precipitates within 1 hour at 37℃, pH-8.

Synthesis of :

• ssRNAs.
• Labeled or unlabeled highly specific RNA probes.
• Capped mRNA using cap analogues.

Greater than 95% as visualized by SDS-PAGE

SDS

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