Recombinant Human Lysozyme

EC 3.2.1.17, LYZ, Lysozyme.

Lysozymeis an antimicrobial enzyme produced by animals that are part of the innate immune system. Lysozyme is a glycoside hydrolase that catalyzes the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan, which is the major component of gram-positive bacterial cell wall.Lysozymes have primarily a bacteriolytic function - those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.

Recombinant Human Lysozyme produced in Plant is a non-glycosylated, polypeptide chain containing 130 amino acids and having a molecular mass of 14kDa. The Recombinant Human Lysozyme is purified by proprietary chromatographic techniques.

Oryza sativa (rice).

Sterile Filtered lyophilized (freeze-dried) off white powder.

The protein (1mg/ml) was lyophilized with no additives.

Stock solutions can be prepared by dissolving gently into PBS for several minutes. Recommended stock concentrations are 1mg/ml in PBS.

Lyophilized Lysozyme although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Lysozyme Human Recombinant should be stored at 4°C between 2-7 days and for future use below -18°C.

For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Please prevent freeze-thaw cycles.

Purity as determined by SDS-PAGE is 85%.

>100,000 Units/mg protein. 1 unit will produce a change of A450 0.001 per minute at pH-6.24 and 25°C, using a suspension of micrococcus lysodeikticus as substrate in a 2.6ml reaction volume.

Recombinant Human Lysozyme is added in lysing bacterial cells to the cell extraction buffer at a range of 0.05-0.5mg/ml (7,000-70,000 U/ml). The common extraction buffer for E. coli cells contains 0.2mg/ml of recombinant Lysozyme in 100mM Tris-HCl, 2mM EDTA, 0.05% Triton X-100, pH 8. The cell paste is suspended in extraction buffer and incubated for at least 15 min at room temperature. Alternatively, Triton X-100 at 1% and PMSF at 1mM can be added separately and the lysis solution is further incubated for at least 15 min at 37C. In other cases, freeze-thaw cycles, sonication, and mechanical cell disruptions are also used to complement the lysozyme lysis protocol.

SDS

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals