- ￥300
- SIGMA
- 美国
- AP504A-1mL
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
瓶身
- 保质期：
coa
- 库存：
999
- 供应商：
麦飞生物
- 规格：
AP504A-1mL
说明
一般描述
IgG is one of the most abundant protein in human serum. IgG is important for our defence against microorganisms and the molecules are produced by B-lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.
特异性
Reacts with with the heavy chain of human IgG. Absorbed for human IgM, IgD and IgA.
The antibody reacts with the heavy chain of human IgG as demonstrated by ELISA and flow cytometry.
免疫原
Epitope: IgG γ chain
Prepared from IgG γ chain. Pooled antisera from goats hyperimmunized with normal human IgG and human IgG paraproteins.
应用
Detect Human IgG γ chain using this Goat anti-Human IgG γ chain Antibody, Alkaline Phosphatase conjugate, Species Adsorbed validated for use in ELISA.
ELISA: 1:2,000-1:4,000.
Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Secondary Antibodies Adsorbed for Dual Labeling
Fragment Specific Secondary Antibodies
外形
Affinity Chromatography
Purified by affinity chromatography on pooled human IgG covalently linked to agarose. Liquid in 50mM Tris/1mM MgCl2/50% Glycerol, pH 8.0, containing 0.1% NaN3 as preservative.
储存及稳定性
Maintain refrigerated at 2°-8°C under sterile conditions for up to twelve months from date of receipt. For long term storage, aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
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文献和实验Class I phosphatidylinositol 3-kinase inhibitor LY294002 activates autophagy and induces apoptosis through p53 pathway in gastric cancer cell line SGC7901
Xing C, et al.
Acta biochimica et biophysica Sinica, 40(3), 194-201 (2008)
Developing Anti-HER2/neu Single-Chain Fv Fragments from Phage Display Libraries
kDa IgG molecule is limited to 1 mm in 2-3 d (4 ). In contrast, smaller molecules such as 50 kDa Fab fragments exhibit faster movement through the tumor interstitium, on the order of 1 to 2 mm per day.
polymerases in the presence of DIG-UTP. These probes were used for hybridization and were immunodetected using anti-DIG antibodies conjugated to horseradish peroxidase. The immunoreactive products were visualized with DAB-H2 O2 . IgG γ-chain mRNA-expressing
Surface plasmon resonance (SPR) is a key technology to evaluate IgG effector functions in vitro involving binding to Fcgamma receptors (FcγR). We describe here the chemical coupling of protein A to a sensor chip. IgGs prepared
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